Co-Expression of hbha and mtb32C Genes from Mycobacterium tuberculosis H37Rv in a Prokaryotic System
نویسندگان
چکیده
منابع مشابه
Construction of a DNA Vaccine Encoding Mtb32C and HBHA Genes of Mycobacterium tuberculosis
BACKGROUND Tuberculosis (TB) is a contagious disease caused by Mycobacterium tuberculosis. Development of a new vaccine for tuberculosis requires immunogenic antigens capable of inducing suitable and long-lasting T cell immunity. The emergence of multidrugs and extensively drug-resistant strains of M. tuberculosis has made it a global public health concern. OBJECTIVES DNA vaccine is a straigh...
متن کاملconstruction of a dna vaccine encoding mtb32c and hbha genes of mycobacterium tuberculosis
conclusions mtb32c and hbha genes were successfully isolated from h37rv genome and cloned into an eukaryotic expression vector. this vector can be considered as a vaccine to evaluate immune responses in animal models. results in the current study, recombinant pcdna3.1 (+) vector containing mtb32c and hbha genes was successfully constructed. the desired size of dna fragment was observed using si...
متن کاملCloning and Expression of Mycobacterium Tuberculosis ESAT-6 in Prokaryotic System
The identification of a large number of antigens with potential for development of new tuberculosis vaccine has been accomplished in recent years. This study was designed for cloning and expression of ESAT-6 as a potent antigen of Mycobacterium tuberculosis. Selected gene (Rv3875) was amplified by PCR and product was ligated into expressing plasmid vector pQE30 and recombinant pQE30-ES plasmi...
متن کاملA study on the immune response induced by a DNA vaccine encoding Mtb32C-HBHA antigen of Mycobacterium tuberculosis
Objective(s): Tuberculosis (TB) has still remained a global health issue. One third of the world's population is infected with tuberculosis and the current BCG vaccine has low efficiency; hence, it is necessary to develop a new vaccine against TB. The aim of the current study was to evaluate the efficiency of a novel DNA vaccine encoding Mtb32C-HBHA antigen in inducing specific immune responses...
متن کاملEvaluation of the eukaryotic expression of mtb32C-hbha fusion gene of Mycobacterium tuberculosis in Hepatocarcinoma cell line
BACKGROUND AND OBJECTIVES HBHA and Mtb32C have been isolated from culture supernatants of Mycobacterium tuberculosis (M. tuberculosis) and Mycobacterium bovis (M. bovis) and their immunogenicity previously studies have been confirmed. In this study, capability of constructed vector containing two mycobacterial immunodaminant antigens (Mtb32C-HBHA), in producing new chimeric protein under the in...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2018
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.14030